G03 Project details
Details of Agency-funded projects under the Safety Assessment Of Novel And GM Food research programme (G03).
An investigation into the use of MudPIT as a tool for the separation and quantification of proteins for GM crop safety assessments
This research project aimed to assess the potential of multidimensional protein identification technology (MudPIT) as quantitative procedure to ascertain protein perturbations arising from genetic modifications.
Results available.
Determination of inauthentic protein glycosylation in transgenic plants
This project sought to develop robust methodologies to characterise the glycosylation of transgenic plant proteins for application in risk assessments.
Results available.
Development of a standard, validated procedure for the isolation of transgene flanking regions in GM crops and detailed analysis of transgene insertion
This project involved the refinement and validation of a rapid polymerase chain reaction (PCR) based method for characterisation of junction sequences in a range of genetically modified (GM) crops.
Results available.
Development of unified data models and data pre-processing strategies for the generation of meaningful, standardised statistical analyses of metabolome variability in crop plants
This project addresses the need to have baseline metabolomic data for crops, to use as a reference in assessing new crop varieties (GM or non-GM). It also addresses how the quality of the raw data used for assessment purposes may affect the safety assessment.
Results available.
Factors influencing transgene survival and transfer in the rumen
This project examined the fate of transgenic DNA in genetically modified (GM) food/feed in the gut of farm animals. In vivo experiments together with in vitro modelling techniques and real-time polymerase chain reaction (PCR) were used to examine DNA survival and the potential for gene transfer to the gut microflora.
Results available.
GMOseek: Development of screening methods for GMOs
In this project nine novel assays/methods to aid the detection of genetically modified organisms (GMOs) in food/feed have been developed. Once successfully validated, the methods will be made available, worldwide, to improve the ability of enforcement laboratories to detect the current and next generation of GMOs present in food/feed.
Results available.
Nanoparticles in food: analytical methods for detection and characterisation
As the production of engineered nanoparticles (ENP) is a relatively new field, there are few established methods for the detection and characterisation of ENP, so there is an urgent need for analytical methods that allow the routine detection of ENP in food.
Post-market monitoring of novel foods: Plant sterols
This research assesses consumption patterns of products containing plant sterols and consumer awareness of labelling on consumption guidelines for these products.
Results available.
Quantitation Using Informative Zeros (QUIZ): proof of concept
This initial proof of concept study is designed to demonstrate the potential of Quantitation Using Informative Zeros (QUIZ) as a high-throughput methodology to measure Genetically Modified (GM) presence in food.
Results available.
Quantitation using informative zeros (QUIZ): part 2 - application for testing GMOs
This project aimed to determine the potential of QUIZ for the quantification of genetically modified (GM) material in food by further development and testing of the methodology established in a previous proof of concept study (G03025).
Results available.
Refinement of GMO screening methods by combining existing multiplex PCR approaches with lab-on-chip capillary electrophoresis endpoint detection
This research project will develop rapid and simple genetically modified organism (GMO) screening methods using multiplex polymerase chain reaction (PCR) approaches and endpoint detection using lab-on-a-chip capillary electrophoresis.
Results available.